全文获取类型
收费全文 | 420篇 |
免费 | 63篇 |
出版年
2021年 | 6篇 |
2018年 | 4篇 |
2017年 | 5篇 |
2016年 | 18篇 |
2015年 | 12篇 |
2014年 | 14篇 |
2013年 | 11篇 |
2012年 | 20篇 |
2011年 | 22篇 |
2010年 | 9篇 |
2009年 | 5篇 |
2008年 | 14篇 |
2007年 | 22篇 |
2006年 | 16篇 |
2005年 | 14篇 |
2004年 | 14篇 |
2003年 | 17篇 |
2002年 | 14篇 |
2001年 | 16篇 |
2000年 | 16篇 |
1999年 | 10篇 |
1998年 | 19篇 |
1997年 | 11篇 |
1996年 | 13篇 |
1995年 | 14篇 |
1994年 | 3篇 |
1993年 | 10篇 |
1992年 | 9篇 |
1991年 | 6篇 |
1990年 | 7篇 |
1989年 | 8篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1986年 | 6篇 |
1985年 | 11篇 |
1984年 | 6篇 |
1982年 | 3篇 |
1981年 | 5篇 |
1980年 | 6篇 |
1979年 | 4篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1971年 | 5篇 |
1969年 | 3篇 |
1967年 | 3篇 |
排序方式: 共有483条查询结果,搜索用时 226 毫秒
11.
12.
L. Mike Conner Steven B. Castleberry Anna M. Derrick 《The Journal of wildlife management》2011,75(4):938-944
Control of mid-sized mammalian predators (hereafter, mesopredators) is sometimes advocated in an attempt to reduce their impact on wildlife populations, particularly economically important (i.e., game) or endangered species. However, mesopredators may play a role in regulating small mammal populations; thus, lethal control of mesopredators may have unintended consequences. The hispid cotton rat (Sigmodon hispidus; hereafter, cotton rat) is one of the most common small mammals in the southeastern United States and is an important prey species for several of the region's predators. Within fire-maintained communities, such as the longleaf pine (Pinus palustris) forests of the Coastal Plain, cotton rat populations dramatically, yet temporarily, decline following prescribed fire. To evaluate the effects of mesopredator removal on cotton rat survival and cause-specific mortality, we conducted a large-scale mesopredator exclusion experiment that incorporated a prescribed fire during the winter of study. Between 18 May 2006 and 20 June 2007, we used radio-telemetry to monitor 252 cotton rats (131 in exclosures and 121 in controls) and documented 184 mortalities. During the 37-week period of monitoring prior to the prescribed fire event, weekly survival of cotton rats was greater in mesopredator exclusion plots. During the 19 weeks following the prescribed fire, there were no differences in weekly survival relative to mesopredator treatment, but fire caused a short-term reduction in weekly survival within both exclosures and controls. Of 36 cotton rats monitored on the date of prescribed fire, 18 were depredated within 1 month, 4 emigrated, and 5 were killed by the fire event. Overall, raptors preyed on cotton rats more in exclosures than in controls, but evidence for compensatory predation (raptor-caused morality greater in exclosures than in controls although survival rates were similar between treatments) only occurred following the prescribed fire event. Our results suggest that managing mesopredators may result in a temporary increase in cotton rat survival, but dormant season prescribed fire removes this effect until well into the following growing season. © 2011 The Wildlife Society. 相似文献
13.
14.
Uri Lopatin Sarah E. Blutt Margaret E. Conner Brian L. Kelsall 《Journal of virology》2013,87(1):524-530
Rotavirus is a major cause of pediatric diarrheal illness worldwide. To explore the role of organized intestinal lymphoid tissues in infection by and immunity to rotavirus, lymphotoxin alpha-deficient (LTα−/−) mice that lack Peyer''s patches and mesenteric lymph nodes were orally infected with murine rotavirus. Systemic rotavirus was cleared within 10 days in both LTα−/− and wild-type mice, and both strains developed early and sustained serum antirotavirus antibody responses. However, unlike wild-type mice, which resolved the intestinal infection within 10 days, LTα−/− mice shed fecal virus for approximately 50 days after inoculation. The resolution of fecal virus shedding occurred concurrently with induction of intestinal rotavirus-specific IgA in both mouse strains. Induction of intestinal rotavirus-specific IgA in LTα−/− mice correlated with the (late) appearance of IgA-producing plasma cells in the small intestine. This, together with the absence of rotavirus-specific serum IgA, implies that secretory rotavirus-specific IgA was produced locally. These findings indicate that serum IgG responses are insufficient and imply that local intestinal IgA responses are important for the clearance of rotavirus from intestinal tissues. Furthermore, they show that while LTα-dependent lymphoid tissues are important for the generation of IgA-producing B cells in the intestine, they are not absolutely required in the setting of rotavirus infection. Moreover, the induction of local IgA-producing B cell responses can occur late after infection and in an LTα-independent manner. 相似文献
15.
Experiments on Xanthium leaf discs were carried out to determine whether concomitant photoreactivation could be detected and if so, to compare its effects with photoreactivation due to post-irradiation treatments. Attempts mere made to simulate certain intensities of unfiltered solar radiation. A small but definite amount of simultaneous reactivation was observed. The dose reduction factor averaged 0.57. It was not clear whether the reactivation was due mainly to direct photoreactivation, photoprotection, or to heating effects. A much larger amount of reactivation due to post-irradiation treatments (direct photoreactivation) was noted with the dose reduction factor averaging 0.30. 相似文献
16.
Holde Puchtler Faye Sweat Waldrop Susan N. Meloan Mary S. Terry H. M. Conner 《Histochemistry and cell biology》1970,21(2):97-116
Summary According to chemical data, methanol raises the shrinkage temperature of collagen significantly more than ethanol (86° C versus 70° C). Since increase of shrinkage temperature appears desirable in tissues to be embedded in paraffin, methanol was substituted for ethanol in Carnoy's fluid. This methanol-Carnoy mixture is referred to as methacarn solution. The fixation-embedding procedure was similar to that described in the study of Carnoy fixation. Methacarn-fixed sections showed little or no shrinkage and compared well with material fixed in Carnoy's or Zenker's fluid. Myofibrils, especially in endothelial and epithelial cells, were more prominent in methacarn- than in Carnoy-fixed tissues.A review of the chemical literature showed that methanol, ethanol and chloroform stabilize or even enhance helical conformations of proteins, presumably by strengthening of hydrogen bonds. Interference with hydrophobic bonds causes unfolding and/or structural rearrangements in globular proteins. The twin-helical structure of DNA collapses in alcoholic solutions. Hence, methacarn fixation can be expected to preserve the helical proteins in myofibrils and collagen, but the conformations of globular proteins and DNA will be significantly altered. Literature on conformational effects produced by fixatives used in electron microscopy was also reviewed. Glutaraldehyde and OsO4 cause considerable loss of helix (22–29% and 39–66% respectively). KMnO4 and glutaraldehyde followed by OsO4 produce extensive transitions from helical to random-coil conformations similar to those seen in powerful denaturants such as 8 M urea. Evidently these fixatives are unsuitable for studies of helical proteins. In contrast ethylene glycol preserves helical conformations. 相似文献
17.
18.
Female arctiid moths rhythmically extrude their ovipositors and thereby rhythmically expose their sex pheromone glands while calling. In Utetheisa ornatrix this behaviour results in concentration-modulated or pulsed pheromone release. The ovipositor protrusion frequencies were determined for 15 arctiid species: U. ornatrix, Haploa clymene, H. colona, H. lecontei, Pyrrarctia isabella, Estigmene acrea, Spilosoma congrua, S. virginica, Ecpantheria scribonia, Phragmatobia fuliginosa, Apantesis nais, A. arge, Pareuchaetes insulata, Cycnia tenera, and Euchaetes egle. Moth species that call early in the scotophase extrude their ovipositors at high frequencies (up to 170.9 ± 24.9 extrusions/min at 25°C) while moth species that call late in scotophase extrude their ovipositors at low frequencies (down to 68.3 ± 5.4 extrusions/min at 25°C). In all species tested, the ovipositor extrusion frequencies were shown to be temperature sensitive with a mean Q10 of 2.0 ± 0.1. In one species, U. ornatrix, the ovipositor extrusion frequency varies with wind speed. In still air the ovipositor extrusion frequency is 70.1 ± 8.4 extrusions/min and at a wind speed of 120 cm/s the ovipositor extrusion frequency is 142.1 ± 8.3 extrusions/min. Suggested functions for rhythmic ovipositor extrusion during calling are discussed. 相似文献
19.
Alternative selectable markers for potato transformation using minimal T-DNA vectors 总被引:4,自引:0,他引:4
Barrell P.J. Yongjin Shang Cooper P.A. Conner A.J. 《Plant Cell, Tissue and Organ Culture》2002,70(1):61-68
Alternative selection systems for plant transformation are especially valuable in clonal crops, such as potato (Solanum tuberosum L.), to pyramid transgenes into the same cultivar by successive transformation events. We have modified the pGPTV series of binary vectors to construct pMOA1 to pMOA5, resulting in a series of essentially identical binary vectors except for the presence of different selectable marker genes. These selectable marker genes are tightly inserted between the left and right T-DNA borders and confer resistance to kanamycin (nptII), hygromycin (hpt), methotrexate (dhfr), phosphinothricin (bar), or phleomycin (ble). The T-DNA of all the vectors is based on the minimal features necessary for plant transformation, with no extraneous DNA segments that may be unacceptable to regulatory authorities for general release of transgenic plants. A series of unique restriction sites exists between the right border and each selectable marker gene for subsequent insertion of useful genes. We have also developed improved culture procedures for potato transformation and used the pMOA1 to pMOA5 binary vectors to define stringent selection conditions for each marker gene. Combining these advances improved the frequency of recovering transformed potato plants while maintaining a low frequency of escapes. The relative efficiency of recovering transgenic potato lines with each selectable marker gene can be summarised as: kanamycin resistance>hygromycin resistance>phosphinothricin resistance>phleomycin resistance>methotrexate resistance. 相似文献
20.
The role of nitric oxide in cancer 总被引:4,自引:0,他引:4
WEIMING XU Li ZHI LIU MARILENA LOIZIDOU MOHAMED AHMED IAN G CHARLES Wolfson Institute for Biomedical Research Cruciform Building Gower Street UCL London WC E AUUK Department of Surgery Charles Bell House - Riding House Street UCL London WW EJ UK 《Cell research》2002,(Z2)
Nitric oxide (NO) is a pleiotropic regulator, critical to numerous biological processes, including va-sodilatation, neurotransmission and macrophage-mediated immunity. The family of nitric oxide synthases (NOS) comprises inducible NOS (iNOS), endothelial NOS (eNOS), and neuronal NOS (nNOS). Interestingly, various studies have shown that all three isoforms can be involved in promoting or inhibiting the etiology of cancer. NOS activity has been detected in tumour cells of various histogenetic origins and has been associated with tumour grade, proliferation rate and expression of important signaling components associated with cancer development such as the oestrogen receptor. It appears that high levels of NOS expression (for example, generated by activated macrophages) may be cytostatic or cytotoxic for tumor cells, whereas low level activity can have the opposite effect and promote tumour growth. Paradoxically therefore, NO (and related reactive nitrogen species) may have both genotoxic and angiogenic pro 相似文献